Experiment 30: Testing impurities in milk
Objective
- To check the milk for impurities
Materials required:
- 20mL milk
- 1% iodine solution
- test tube
- soybean/ arhar powder
- red litmus paper
- dil. sulphuric acid
Procedure - Test for Starch
- Add a few drops of tincture of Iodine or Iodine solution.
- Formation of blue colour indicates the presence of starch.
Procedure - Test for Urea
- Take a teaspoon of milk in a test tube.
- Add ½ teaspoon of soybean or arhar powder.
- Mix up the contents thoroughly by shaking the test tube.
- After 5 minutes, dip a red litmus paper in it.
- Remove the paper after ½ a minute.
- A change in colour from red to blue indicates the presence of urea in the milk.

Procedure - Test for Formalin
- Take 10 ml of milk in a tests tube and add 5 ml of conc. sulphuric acid from the sides of the wall without shaking.
- If a violet or blue ring appears at the intersection of two layers then it shows presence of formalin.

Observations and Findings:
- After the solvent has risen about 15 cm you will notice two different spots of blue and red colors on the filter paper. The pattern is called chromatograph.
Conclusions:
- Over time, we see a pattern composed of various colors / components of the ink

Theory:
- Household milk generally contains starch an impurity. In some cases, it might have urea or formalin (added to increase shelf life of milk).